Application of biophysical methods for improved protein production and characterization: a case study on an HtrA-family bacterial protease
M. H. Ronzetti, B. Baljinnyam, I. Jalal, U. Pal, A. Simeonov
This paper improves the production and characterization of HtrA-family proteins using optimized expression, refolding, and biophysical methods, resulting in pure, active proteins with monodisperse composition.
bioRxiv · Jan 27, 2022Biochemistry