Highly Efficient Knockin in Human iPS Cells and Rat Embryos by CRISPR/Cas9 Molecular Optimization
V. Chenouard, I. Leray, L. Tesson, S. Remy, A. Fortun, K. Bernardeau, Y. Cherifi, L. David, I. Anegon
This study optimizes CRISPR/Cas9 reagent concentrations for efficient knockin in human iPS cells and rat embryos, achieving 50% knockin efficiency with reduced costs and toxicity.
bioRxiv · Sep 3, 2021 · PreprintBioengineering