A general method to quantify ligand-driven oligomerization using single- or two-photon excitation microscopy
M. R. Stoneman, G. Biener, R. J. Ward, J. D. Pediani, D. N. Badu, I. V. Popa, G. Milligan, V. Raicu
This paper introduces a method to quantify ligand-driven oligomerization using fluorescence intensity fluctuation spectrometry with single- or two-photon excitation microscopy, showing it can detect oligomer size and stability in membrane proteins.
bioRxiv · Nov 23, 2018 · PreprintBiophysics