V. Raicu

Impact of photobleaching of fluorescent proteins on FRET measurements under two-photon excitation

This study investigates how photobleaching affects FRET measurements under two-photon excitation and provides guidelines for reliable FRET experiments.

The efficacy of EphA2 tyrosine phosphorylation increases with EphA2 oligomer size

This study investigates the relationship between EphA2 oligomer size and signaling efficacy, finding a correlation between mean oligomer size and tyrosine phosphorylation.

In-cell detection of conformational sub-states of a GPCR quaternary structure: Modulation of sub-state probability by cognate ligand binding

This study improves FRET spectrometry to detect conformational sub-states of GPCR oligomers and shows ligand binding modulates these sub-states in Ste2.

Reply To: Molecular Brightness analysis of GPCR oligomerization in the presence of spatial heterogeneity

The paper replies to a suggestion for improving 2D FIF spectrometry by showing that its built-in filtering already handles spatial heterogeneity effectively.

A general method to quantify ligand-driven oligomerization using single- or two-photon excitation microscopy

This paper introduces a method to quantify ligand-driven oligomerization using fluorescence intensity fluctuation spectrometry with single- or two-photon excitation microscopy, showing it can detect oligomer size and stability in membrane proteins.

Ab initio derivation of the FRET equations resolves old puzzles and suggests measurement strategies

The paper derives FRET equations from kinetic models to resolve experimental puzzles and guide measurement strategies.