Amplicon sequencing of the 16S-ITS-23S rRNA operon with long-read technology for improved phylogenetic classification of uncultured prokaryotes
Joran Martijn, Anders E. Lind, Ian Spiers, Lina Juzokaite, Ignas Bunikis, Olga Vinnere Pettersson, Thijs J.G Ettema
The paper develops a method using long-read sequencing to improve phylogenetic classification of uncultured prokaryotes by amplifying and sequencing the 16S-ITS-23S rRNA operon.
bioRxiv · Dec 15, 2017 · PreprintMicrobiology