Ignas Bunikis

A national ecosystem for long-read genome sequencing: Integrating population genomics and rare disease diagnostics in Sweden

This paper evaluates the clinical use of long-read genome sequencing in Sweden, showing it provides molecular diagnoses and reduces diagnostic costs.

Female-biased sex ratios despite stable genetic sex determination across a climatic gradient in a marine fish

The paper investigates adult sex ratio bias in a marine fish across a climatic gradient, finding female bias arises from ecological factors rather than genetic or environmental sex determination.

Accurate characterization of CRISPR-Cas9 genome editing outcomes and mosaicism with near-perfect long reads

This study uses long-read sequencing to analyze CRISPR-Cas9 editing outcomes and mosaicism in zebrafish, revealing genetic mosaicism in founder fish and its inheritance to offspring.

Genomic studies in Linum shed light on the evolution of the distyly supergene and the molecular basis of convergent floral evolution

This study examines the evolution of the S-locus supergene and molecular mechanisms of distyly in Linum species, finding conserved genetic architecture and brassinosteroid pathway involvement in style length polymorphism.

Limited parallelism in genetic adaptation to brackish water bodies in European sprat and Atlantic herring

The paper analyzes genetic adaptation in European sprat and Atlantic herring to brackish waters, finding limited parallelism in genetic changes despite similar environmental pressures.

Long-read whole genome analysis of human single cells

This study uses long-read sequencing to analyze the genomic architecture of single human cells, revealing high-resolution genetic variation and structural variants.

Amplification-free long read sequencing reveals unforeseen CRISPR-Cas9 off-target activity

The paper introduces amplification-free long-read sequencing protocols to detect CRISPR-Cas9 off-target binding sites, identifying many previously undetected sites that are not predicted by existing software.

Identifying the causes and consequences of assembly gaps using a multiplatform genome assembly of a bird-of-paradise

This study evaluates sequencing technologies and assembly strategies to improve genome completeness in a non-model organism, focusing on resolving dark matter regions like transposable elements and GC-rich areas.

The population genomics of structural variation in a songbird genus

The paper characterizes structural variation in the songbird genus Corvus using multiple sequencing methods and identifies a retrotransposon insertion with evolutionary implications.

A chromosome-level assembly of the Atlantic herring - detection of a supergene and other signals of selection

The paper presents a chromosome-level genome assembly of Atlantic herring, enabling the detection of a supergene and improved analysis of selection signals.

A hybrid de novo genome assembly of the honeybee, Apis mellifera, with chromosome-length scaffolds

The paper presents a new honeybee genome assembly using hybrid sequencing technologies, resulting in a more contiguous and complete genome with chromosome-length scaffolds.

De novo assembly of two Swedish genomes reveals missing segments from the human GRCh38 reference and improves variant calling of population-scale sequencing data

The paper performs de novo assembly of two Swedish genomes, identifies novel sequences absent from GRCh38, and shows improved variant calling with these sequences.

Amplicon sequencing of the 16S-ITS-23S rRNA operon with long-read technology for improved phylogenetic classification of uncultured prokaryotes

The paper develops a method using long-read sequencing to improve phylogenetic classification of uncultured prokaryotes by amplifying and sequencing the 16S-ITS-23S rRNA operon.