Evaluation of established methods for DNA extraction and primer pairs targeting 16S rRNA gene for bacterial microbiome profiling of olive xylem sap
C. HARO, M. ANGUITA-MAESO, M. Metsis, J. A. NAVAS-CORTES, B. LANDA
This study evaluates DNA extraction kits and 16S rRNA gene primer pairs for accurately profiling bacterial communities in olive xylem sap, finding that specific kits and primers significantly affect community composition and diversity metrics.
bioRxiv · Dec 11, 2020Microbiology