Widening the bottleneck: Heterologous expression, purification, and characterization of the Ktedonobacter racemifer minimal type II polyketide synthase in Escherichia coli
J. G. Klein, Y. Wu, B. Kokona, L. K. Charkoudian
This study expresses, purifies, and characterizes minimal type II PKS components from Ktedonobacter racemifer in E. coli, revealing self-malonylating activity of KrACP and no in vitro interaction between KrACP and KrKSCLF.
bioRxiv · May 18, 2020Biochemistry