Author

B. Kokona

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Biochemistry· 4

Signature Assays, Organisms & Methods

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Approach:experimental assay· 4 Research Focus:protein folding & dynamics· 3 Research Focus:cell type annotation· 2 Organism:E. coli· 2 Assay:proteomics· 2 Organism:bacteria (other)· 1 Approach:genetic perturbation / CRISPR· 1 Organism:mouse· 1

Frequent Co-Authors

L. K. Charkoudian· 3 shared Y. I. Cho· 2 shared A. E. Connolly-Sporing· 1 shared A. Kang· 1 shared A. Sulpizio· 1 shared B. T. DeRose· 1 shared C. A. Daly· 1 shared C. E. W. Crawford· 1 shared

Activity by Year

2020-2023 · peak 2/yr

Co-Author Collaboration Network

10 top collaborators · 36 mutual co-author links

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3 shared papersBiochemistry

L. K. Charkoudian

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4 papers

Recent·Most cited

Strategic acyl carrier protein engineering enables functional type II polyketide synthase reconstitution in vitro

K. K. Li, Y. I. Cho, M. A. Tran, C. Wiedemann, S. Zhang, R. S. Koweek, N. K. Hoang, G. Hamrick, M. Bowen, B. Kokona, P. Stallforth, J. Beld, U. Hellmich, L. K. Charkoudian

This study enables functional reconstitution of type II PKS in vitro by engineering acyl carrier proteins to be activatable by Sfp.

bioRxiv · Aug 2, 2023Biochemistry

Engineered chimeras unveil swappable modular features of fatty acid and polyketide synthase acyl carrier proteins

Y. I. Cho, C. L. Armstrong, A. Sulpizio, K. K. Acheampong, K. N. Banks, O. Bardhan, S. J. Churchill, A. E. Connolly-Sporing, C. E. W. Crawford, P. L. Cruz Parrilla, S. M. Curtis, L. M. De La Ossa, S. C. Epstein, C. J. Farrehi, G. S. Hamrick, , L. K. Charkoudian

This study designs chimeric acyl carrier proteins to swap modular features between different ACPs, enabling compatibility with specific enzymes for synthetic pathway engineering.

bioRxiv · Dec 30, 2021Biochemistry

Production and analysis of a mammalian septin hetero-octamer complex

B. T. DeRose, R. S. Kelley, R. Ravi, B. Kokona, E. T. Spiliotis, S. B. Padrick

This paper produces and characterizes a recombinant mammalian septin hetero-octamer complex, revealing its structure and polymerization behavior.

bioRxiv · Jun 15, 2020Biochemistry

Widening the bottleneck: Heterologous expression, purification, and characterization of the Ktedonobacter racemifer minimal type II polyketide synthase in Escherichia coli

J. G. Klein, Y. Wu, B. Kokona, L. K. Charkoudian

This study expresses, purifies, and characterizes minimal type II PKS components from Ktedonobacter racemifer in E. coli, revealing self-malonylating activity of KrACP and no in vitro interaction between KrACP and KrKSCLF.

bioRxiv · May 18, 2020Biochemistry