Cross-Genera Amplification of prs/hlyA by Multiplex PCR Resulted in Misidentification of Enterococcus faecium as Listeria monocytogenes
H. B. Ali, A. S Kumurya, J. M. Ajagbe, Yahaya Usman, Abubakar Sadiq Baba, M Usman
The study found that a common PCR assay for Listeria monocytogenes misidentified Enterococcus faecium as L. monocytogenes due to primer cross-reactivity, highlighting a 66.7% false-positive rate in a Nigerian context.
bioRxiv · Jan 21, 2026 · PreprintMicrobiology