Transcriptome-wide analysis of the function of Ded1 in translation preinitiation complex assembly in a reconstituted in vitro system
F. Zhou, J. M. Bocetti, M. Hou, D. Qin, A. G. Hinnebusch, J. R. Lorsch
This study uses Rec-Seq to show Ded1 promotes 48S PIC formation on mRNAs with long 5UTRs and that eIF4A is required for PIC assembly on all mRNAs.
bioRxiv · Oct 16, 2023Biochemistry