J. N. Milstein

Enhancing the Applied Force and Range of Axial Optical Tweezers

This paper improves axial optical tweezers by extending force range and reducing laser power through aberration correction and nonlinear response accounting.

Spatial exclusion leads to tug-of-war ecological dynamics between competing species within microchannels

This paper extends the Moran model to include spatial exclusion in microchannels, showing it alters fixation probabilities and fixation times compared to classical models.

Quantitative Single-Molecule Imaging with Statistical Machine Learning

The paper develops a machine learning method to quantify macromolecular complexes in SMLM data without requiring calibration.

Estimating the Dynamic Range of Quantitative Single-Molecule Localization Microscopy

This study defines and quantifies the dynamic range of single-molecule localization microscopy by analyzing photophysical and experimental factors affecting molecule counting accuracy.

An Ultra-Stable and Dense Single-Molecule Click Platform for Sensing Protein-DNA Interactions

This paper introduces an improved single-molecule assay for studying protein-DNA interactions, demonstrating enhanced stability and compatibility with Mg2+.

Pinhole Optical Tweezers: Extending the Photobleaching Lifetime in the Presence of an Optical Trap by Wavefront Engineering

The paper introduces a pinhole optical trap that extends photobleaching lifetime by separating trap light from fluorescence illumination using wavefront engineering.

Nanoscopic Stoichiometry and Single-Molecule Counting

This paper introduces a method for molecular counting using single-molecule localization microscopy (SMLM) and benchmarks it on DNA origami grids to demonstrate its accuracy for optical -omics analysis.

Polarization effects and the calibration of a donut beam axial optical tweezers for single-molecule force spectroscopy

This paper describes the calibration of a donut beam optical tweezers for single-molecule force spectroscopy, addressing polarization effects and trap height precision.

Molecular Counting with Localization Microscopy: A Bayesian estimate based on single fluorophore statistics

The paper develops a Bayesian method for molecular counting using localization microscopy, showing improved accuracy with multiple fluorophore labeling and applying it to quantify plasmid copy number variability in bacteria.