Profiling Expression Strategies for a Type III Polyketide Synthase in a Lysate-Based, Cell-free System
Tien T. Sword, Jaime Lorenzo N. Dinglasan, Ghaeath S. K. Abbas, J. William Barker, Madeline E. Spradley, Elijah R. Greene, Damian S. Gooden, Scott Emrich, Michael A. Gilchrist, Mitchel J. Doktycz, Constance B. Bailey
This paper improves the expression of a type III polyketide synthase in cell-free systems by optimizing codon usage and promoters, showing better results than traditional codon optimization.
Scientific Reports · Jun 5, 2024Peer-reviewedSynthetic Biology