Jaime Lorenzo N. Dinglasan

Profiling Expression Strategies for a Type III Polyketide Synthase in a Lysate-Based, Cell-free System

This paper improves the expression of a type III polyketide synthase in cell-free systems by optimizing codon usage and promoters, showing better results than traditional codon optimization.

A Lysate Proteome Engineering Strategy for Enhancing Cell-Free Metabolite Production

This paper describes a method to enhance cell-free metabolite production by engineering crude lysates using MAGE to selectively remove proteins, resulting in increased pyruvate production.