Optimizing nucleic acid extraction and transcriptome evaluation from low-input, fixed clinical samples
Katie Marie Campbell, Egmidio Medina, Ignacio Baselga-Carretero, Yaroslav Teper, Rangasamy P. Elumalai, Raymond Miller, Kasturi Pal, Jessica Pitzer, Eugenio Daviso, Carsten Carstens, James A. Laugharn, Willy Hugo, Jia Ming Chen, Agustin Vega-Crespo, Sameeha Jilani, , Antoni Ribas
The paper evaluates nucleic acid extraction kits and RNA sequencing library preparations for FFPE clinical samples, finding that combinations of methods affect transcriptome analysis.
bioRxiv · Jun 2, 2020 · PreprintGenomics