CRISPR-Cas9 enables efficient genome engineering of the strictly lytic, broad host-range staphylococcal bacteriophage K
Jonas Fernbach, Jasmin Baggenstos, Aleksandra Svorjova, Jeannine Riedo, Shawna E. McCallin, Martin J. Loessner, Samuel Kilcher
This paper develops a CRISPR-Cas9-based platform for engineering Twortvirinae phages to create reporter phages for detecting Staphylococcus aureus in clinical and environmental samples.
Applied and Environmental Microbiology · Aug 4, 2025Peer-reviewedBioengineering