Analytical and functional clearance of dsRNA contaminants from in vitro transcribed mRNA by an engineered dsRNA-binding protein
Tyson Vonderfecht, Peter Lam, Genia Verovskaya, Jason Potter
This paper identifies dsRNA contaminants in IVT mRNA, shows their functional impact, and introduces B2-S, an engineered protein to clear them, improving mRNA quality and reducing immunostimulatory effects.
bioRxiv · Sep 7, 2026 · PreprintBioengineering