A versatile in situ cofactor enhancing system for meeting cellular demands for engineered metabolic pathways
Juthamas Jaroensuk, Chalermroj Sutthaphirom, Jittima Phonbuppha, Wachirawit Chinantuya, Chatchai Kesornpun, Nattanon Akeratchatapan, Narongyot Kittipanukul, Kamonwan Phatinuwat, Sopapan Atichartpongkul, Mayuree Fuangthong, Thunyarat Pongtharangkul, Frank Hollmann, Pimchai Chaiyen
This paper demonstrates that increasing sugar phosphates using the XR/lactose system enhances cofactor generation in engineered metabolic pathways in E. coli, improving productivities by 2-4-fold.
bioRxiv · Jan 8, 2023 · PreprintSynthetic Biology