John A. Ray

Comparative analysis of convergent and divergent T7 RNA polymerase promoters for the synthesis of dsRNA in vivo and in vitro

This study compares convergent and divergent T7 RNA polymerase promoters for dsRNA synthesis in vitro and in vivo, finding that divergent promoters yield higher dsRNA for smaller transcripts and in vitro.

Optimising the production of dsRNA biocontrols in microbial systems using multiple transcriptional terminators.

This study optimizes dsRNA production in E. coli using synthetic transcriptional terminators, achieving higher yields and demonstrating scalability and efficacy in insect mortality.