Mark J. Dickman

Multi-attribute characterisation of mRNA via MazF endoribonuclease and LC-MS workflows

The paper presents two LC-MS workflows using MazF endoribonuclease to characterise mRNA sequence identity, 5' capping efficiency, and poly(A) tail length and heterogeneity.

DNA template heterogeneity and in vitro transcription reaction conditions impact the poly(A) tail length and heterogeneity of mRNA

A Universal, AI-based Design Framework for Efficient Manufacturing of mRNA Therapeutics

This paper introduces an AI framework to improve mRNA therapeutic manufacturing by predicting and optimizing sequence design for in vitro transcription efficiency.

Comparative analysis of convergent and divergent T7 RNA polymerase promoters for the synthesis of dsRNA in vivo and in vitro

This study compares convergent and divergent T7 RNA polymerase promoters for dsRNA synthesis in vitro and in vivo, finding that divergent promoters yield higher dsRNA for smaller transcripts and in vitro.

The evolution and developmental dynamics of histone-based chromatin regulation in Annelida

This study investigates histone-based chromatin regulation in Annelida using multi-omics approaches, revealing lineage-specific effects on histone repertoires and functional implications of histone variants.

GRASPS: a simple-to-operate translatome technology reveals omics-hidden disease-associated pathways in TDP-43-related amyotrophic lateral sclerosis

GRASPS identifies more differentially expressed transcripts with higher fold changes and significance in TDP-43 ALS models, revealing previously missed disease pathways.

Optimising the production of dsRNA biocontrols in microbial systems using multiple transcriptional terminators.

This study optimizes dsRNA production in E. coli using synthetic transcriptional terminators, achieving higher yields and demonstrating scalability and efficacy in insect mortality.

The master energy homeostasis regulator PGC-1alpha couples transcriptional co-activation and mRNA nuclear export

This study identifies a novel RNA-binding function of PGC-1alpha that couples transcriptional co-activation with mRNA nuclear export and mitochondrial homeostasis.

hnRNPUL1 ensures efficient Integrator-mediated cleavage of snRNAs and is mutated in amyotrophic lateral sclerosis

This study identifies hnRNPUL1's role in Integrator-mediated snRNA cleavage and links its mutations to amyotrophic lateral sclerosis.

Pneumolysin is responsible for differential gene expression and modifications in the epigenetic landscape of primary monocyte derived macrophages.

This study shows pneumolysin alters gene expression and histone modifications in macrophages, dampening innate immune responses through epigenetic mechanisms.

A proteome-wide screen reveals widespread roles for the phosphatase Cdc14 in the Candida albicans cell cycle

The paper identifies 126 proteins interacting with Cdc14 in C. albicans, revealing its role in cell cycle regulation and unexpected connections to ergosterol biosynthesis.