Histological techniques for the visualisation and identification of polymicrobial communities in mucosal tissue
Harriet J. Giddings, Ana Teodòsio, Jordanne Jones, William A. Butterworth, Claire D Shannon-Lowe, Jeffrey A. Cole, Amanda E. Rossiter
This study validates a modified Gram stain technique to identify mucosal tissue with high bacterial biomass for downstream microbiota analysis, improving reproducibility and reducing noise in sequencing studies.
bioRxiv · Nov 26, 2025 · PreprintMicrobiology