Ian R. Henderson

Transposon mutagenesis uncovers the genetic landscape of streptomycin susceptibility and implicates SbmA in aminoglycoside uptake in Escherichia coli

This study uses transposon mutagenesis in E. coli to identify genes affecting streptomycin susceptibility, revealing SbmA's role in aminoglycoside uptake.

Vi-TIS: a transposon insertion sequencing framework for quantitative discrimination of viable and non viable mutants

Vi-TIS improves TIS accuracy by excluding dead cells using PMA, enhancing essential gene identification and revealing new antibiotic susceptibility patterns in E. coli.

SeaB is a conserved Salmonella enterica extracellular matrix binding protein involved in biofilm formation and infection

This study characterizes SeaB, a Type 5a secretion system protein in Salmonella enterica, showing its role in biofilm formation, virulence, and protective immunity in a mouse model.

Vaccine-induced antibodies are sufficient to limit Salmonella infection in the absence of complement or macrophages.

This study shows that antibodies alone can limit Salmonella infection, but their effectiveness is enhanced by complement and macrophages.

Genome scale identification of new genes using saturated reporter transposon mutagenesis

The paper identifies new protein-coding sequences in E. coli using transposon mutagenesis and translation reporters, revealing previously unannotated genes.

Extracellular loops of the β-barrel domain catalyze rapid folding for function of self-associating autotransporters

This study shows that extracellular loops of the β-barrel domain in Ag43 are essential for passenger domain folding and bacterial aggregation.

The gatekeeper to gastric cancer; gastric microbiota invade the lamina propria in Helicobacter pylori-associated gastric carcinogenesis

This study investigates the spatial organisation of gastric microbiota in health and disease, finding that non-H. pylori bacteria invade the lamina propria in H. pylori-associated gastric carcinogenesis.

A scalable, multi-resolution consensus clustering approach for prioritising robust signals from high-throughput screens

This paper introduces a consensus clustering method to identify robust signals from high-throughput screens by analyzing multi-scale data organization in cancer cell lines and bacterium models.

Wild-type and single-O-antigen repeat outer-membrane vesicles induce equivalent protection against homologous and heterologous Salmonella challenge

This study shows that OMVs with different O-antigen lengths induce similar protection against Salmonella infections, and boosting enhances cross-protection.

TraDIS-validate: a method for curating ordered gene-replacement libraries

TraDIS-validate is a method to validate ordered gene-replacement libraries, achieving 99.7% accuracy in identifying correct kanamycin cassette positions in the Keio library.

AscA (YecA) is a molecular chaperone involved in Sec-dependent protein translocation in Escherichia coli

This paper identifies AscA (YecA) as a novel molecular chaperone that facilitates Sec-dependent protein translocation in E. coli by interacting with SecB and ribosomes.

The role of a single non-coding nucleotide in the evolution of an epidemic African clade of Salmonella

The paper identifies a non-coding SNP in the pgtE promoter of Salmonella ST313 that increases PgtE expression, contributing to serum resistance and virulence in a chicken model.

Mammalian Cell Entry domains are required for bile resistance and virulence in Salmonella

This study shows that MCE domains in Salmonella Typhimurium are essential for bile resistance and virulence, not for cell entry or adherence.