An optimized protocol for single-brain isolation of sex-defined pure mouse astrocyte cultures
Sanarya Al-Jaf, En-Hung Ai, Julia A. Wilson, Khaled S. Abd‐Elrahman
This paper introduces a protocol to generate sex-specific, high-purity astrocyte cultures from single neonatal mouse brains, avoiding the need for tissue pooling.
bioRxiv · Mar 25, 2026 · PreprintPharmacology & Toxicology