Inferring cellular and molecular processes in single-cell data with non-negative matrix factorization using Python, R, and GenePattern Notebook implementations of CoGAPS
Jeanette Johnson, Ashley P. Tsang, Jacob T. Mitchell, David L. Zhou, Julia Bowden, Emily F. Davis-Marcisak, Thomas D. Sherman, Ted Liefeld, Melanie Loth, Loyal A. Goff, Jacquelyn W. Zimmerman, Ben Kinny-Köster, Elizabeth M. Jaffee, Pablo Tamayo, Jill P. Mesirov, , Genevieve Stein-O’Brien
The paper introduces computational tools for NMF analysis of single-cell RNA-seq data, demonstrating their use in quantifying cell state transitions in pancreatic cancer.
Nature Protocols · Nov 21, 2023Peer-reviewedSystems Biology