Optimization of gene knockout approaches and practical solutions to sgRNA selection challenges in hPSCs with inducible Cas9 system
Jie Ni, Junhao Gong, Yuqing Ran, Rui Bai, Pufeng Huang, Zihe Zheng, Meng Ran Zhou, Feng Lan, Wei Gu, Xujie Liu
This study optimizes gene knockout in hPSCs using an inducible Cas9 system and identifies effective sgRNAs by comparing experimental results with cleavage efficiency algorithms.
Scientific Reports · Nov 18, 2025Peer-reviewedCell Biology