Permethylation of ribonucleosides provides enhanced mass spectrometry quantification of post-transcriptional modifications
Y. Xie, K. A. Janssen, A. Scacchetti, R. Bonasio, B. A. Garcia
This paper introduces a method to enhance RNA modification analysis using permethylation and LC-MS/MS, showing improved detection of cytosine methylation and hydroxymethylation in mouse embryonic stem cells.
bioRxiv · Jan 26, 2022Biochemistry