Coupling microdroplet-based sample preparation, multiplexed isobaric labeling, and nanoflow peptide fractionation for deep proteome profiling of tissue microenvironment
M. Velickovic, T. L. Fillmore, K. Attah, C. Posso, J. C. Pino, R. Zhao, S. M. Williams, D. Velickovic, J. M. Jacobs, K. E. Burnum-Johnson, Y. Zhu, P. D. Piehowski
This paper develops a combined proteomic workflow to enable deep spatial proteome profiling of small tissue samples, achieving over 5,000 protein quantifications from a human pancreatic tissue pixel.
bioRxiv · Mar 13, 2023 · PreprintBiochemistry