Base editor scanning charts the DNMT3A activity landscape
N. Z. Lue, E. M. Garcia, K. C. Ngan, C. Lee, J. G. Doench, B. B. Liau
The paper uses base editing and a DNA methylation reporter to scan DNMT3A mutations, identifying functional perturbations and revealing a noncanonical DNA affinity role for the PWWP domain.
bioRxiv · Apr 12, 2022Biochemistry