Establishment of a simplified preparation method for single-nucleus RNA-sequencing and its application to long-term frozen tumor tissues
K. F. Ernst, K. Okonechnikov, J. Bageritz, J.-P. Mallm, A. Wittmann, K. K. Maass, S. Leible, M. Boutros, S. M. Pfister, M. Zuckermann, D. T. W. Jones
The paper develops a simplified method for isolating nuclei from frozen tumor tissues and applies it to analyze pediatric central nervous system tumors using snRNA-seq.
bioRxiv · Oct 23, 2020 · PreprintCancer Biology