Choice of library preparation and its effects on sequence quality, genome assembly, and precise in silico prediction of virulence genes in shiga toxin producing Escherichia coli
J. Haendiges, K. Jinneman, N. Gonzalez-Escalona
This study compares Nextera XT and Nextera DNA Prep library prep kits for sequencing STEC strains, finding that Nextera DNA Prep provides better sequence quality, genome assembly, and virulence gene detection.
bioRxiv · Nov 2, 2020Microbiology