HuluFISH non-denaturing in situ detection of genomic DNA opened by CRISPR-Cas9 Nickase and Exonuclease
Kang Han, Sheng Liu, Yong-Sheng Cheng
The paper introduces a method to detect small genomic loci using CRISPR-Cas9 nickase and exonuclease to open DNA for FISH without heat denaturation.
bioRxiv · Dec 23, 2021 · PreprintGenomics