Thermal optimized PCR coupled to CRISPR-Cas12a for rapid detection of blaOXA-1 resistance gene
Ana Quiroz-Huanca, Maryhory Vargas-Reyes, J. Lopez, Kiara Flores-Jimenez, Sofia Saldarriaga-Morán, Karla Cifuentes, Roberto Alcántara
This paper optimizes a PCR-CRISPR-Cas12a assay for rapid detection of the blaOXA-1 gene, achieving results in under an hour with basic equipment.
PLoS ONE · May 15, 2026Peer-reviewedMolecular Biology