Roberto Alcántara

This study identifies optimal buffer conditions for LbCas12a trans-cleavage activity, showing that while some buffers enhance catalytic efficiency, they also increase nonspecific cleavage, requiring careful optimization for detection performance.

The paper develops a portable fluorescence platform for decentralized monitoring of mcr-1, achieving high sensitivity and concordance with sequencing and phenotypic tests.

This paper optimizes a PCR-CRISPR-Cas12a assay for rapid detection of the blaOXA-1 gene, achieving results in under an hour with basic equipment.

This study develops C12a, a CRISPR/Cas12a-based tool for detecting antibiotic resistance markers with high sensitivity and concordance across multiple assays.