Detection of specific uncultured bacteriophages by fluorescence in situ hybridisation in pig microbiome
L. Jensen Ostenfeld, P. Munk, F. M. Aarestrup, S. Otani
The paper detects specific uncultured bacteriophages in pig microbiomes using modified phageFISH and PCR, revealing their life cycle stages and demonstrating the first in vivo detection of jumbophages without culturing.
bioRxiv · Feb 18, 2022 · PreprintMicrobiology