L. Witherspoon

Deep Learning-based Automated Rare Sperm Identification from Testes Biopsies

The paper develops a CNN to detect rare sperm in testis biopsies, achieving an 85.2% F1 score, which could improve IVF-ICSI success rates for NOA patients.

A Xeno-free Media for the In Vitro Expansion of Human Spermatogonial Stem Cells

This study develops a xeno-free media for expanding human spermatogonial stem cells using PGD-2 and IGF-1, showing improved viability and marker expression compared to animal-derived components.

Differentiation of Peritubular Myoid-Like Cells from Human Induced Pluripotent Stem Cells

This study generates peritubular myoid-like cells from human induced pluripotent stem cells by modifying a Leydig cell differentiation protocol, enabling in vitro study of spermatogenesis.

Microfluidic bioprinting for the in vitro generation of novel biomimetic human testicular tissues

This study uses 3D bioprinting to create biomimetic human testicular tissues and demonstrates that printed cells retain viability and reorganize into native cytoarchitecture.

A Novel Organoid Model of In Vitro Spermatogenesis Using Human Induced Pluripotent Stem Cells

This study develops a human testicular organoid model from hiPSCs that supports spermatogenesis up to the post-meiotic spermatid stage, offering an alternative to scarce primary testicular tissues.