dCas allele sequestration (das-CRISPR): A Versatile New Method to Achieve Monoallelic Gene Editing in Mouse Embryos and in cell culture.
Ghassan Yehia, Jian Pan, Laura Servinsky, Xuening Hong, Peter J. Romanienko
This paper introduces das-CRISPR, a method to achieve monoallelic gene editing in mouse embryos and cell culture by using dCas9 and Cas9 RNPs with the same sgRNA.
bioRxiv · Jun 4, 2026 · PreprintMolecular Biology