Evolutionary BiologybioRxiv
High throughput sequencing of multiple amplicons for barcoding and integrative taxonomy
Perrine Cruaud, Jean-Yves Rasplus, Lillian Jennifer Rodriguez, Astrid Cruaud
The paper develops a cost-effective method using MiSeq sequencing to simultaneously sequence multiple markers from hundreds of specimens, showing high success rates and highlighting the need for quality control and multiple markers in DNA-taxonomy.
DOI 10.1038/srep41948