Rational Design of Evolutionarily Stable Microbial Kill Switches
Finn Stirling, Lisa Bitzan, Samuel O’Keefe, Elizabeth S. P. Redfield, John W. K. Oliver, Jeffrey C. Way, Pamela A. Silver
This paper designs and tests two evolutionarily stable kill switches in E. coli, showing they maintain functionality for over 140 generations in vitro and limit escape in vivo.
Molecular Cell · Nov 1, 2017Peer-reviewedSynthetic Biology