Gene editing without a genome: generation and validation of F0 CRISPR mutants in gastropod mollusc Crepidula fornicata
Danielle C. Jordan, Molly L. Rivers, Lucy Belmont, Samuel Martín, Tim P. Bean, Victoria A. Sleight
The paper generates and validates F0 CRISPR-Cas9 pax6 knockout mutants in the gastropod Crepidula fornicata without an assembled genome, showing eye-loss phenotype and confirming edits via Sanger sequencing and ICE analysis.
Developmental Biology Advances · May 14, 2026Peer-reviewedDevelopmental Biology