Protein semi-synthesis enables real-time optical tracking of intracellular conformational changes during sodium channel inactivation
Laurie Peverini, Michelle Nilsson, Iacopo Galleano, Vita Sereika-Bejder, Emma Krindel Beyer, Lukas Fagerlund, Janne M. Colding, Gerbrand J. van der Heden van Noort, Kristian Strømgaard, Stephan Alexander Pless
This study uses protein semi-synthesis and voltage-clamp fluorometry to track real-time conformational changes in the cardiac sodium channel NaV1.5 during inactivation.
bioRxiv · Aug 25, 2026 · PreprintBiophysics