Streamlined, cell-specific single-molecule RNA-FISH of core clock mRNAs in clock neurons in whole mount Drosophila brains
Y. Yuan, M. A. Padilla, D. Clark, S. Yadlapalli
The paper introduces a streamlined smFISH protocol and machine learning analysis to quantify Clock mRNA levels and localization in Drosophila clock neurons, showing cyclic mRNA counts in lLNvs.
bioRxiv · Sep 5, 2022Cell Biology