Demonstration of electron diffraction from membrane protein crystals grown in a lipidic mesophase after lamella preparation by focused ion beam milling at cryogenic temperatures
V. Polovinkin, K. P. Khakurel, M. Babiak, B. Angelov, B. Schneider, J. Dohnalek, J. Andreasson, J. Hajdu
This paper demonstrates that cryo-FIB milling can remove the viscous lipidic matrix around membrane protein crystals, enabling high-quality electron diffraction for 3D crystallography.
Journal of Applied Crystallography · Dec 1, 2020Peer-reviewedBiophysics