An NMR-based biosensor to measure stereo-specific methionine sulfoxide reductase (MSR) activities in vitro and in vivo.
M. C. Sanchez-Lopez, N. Labadie, V. A. Lombardo, F. A. Biglione, B. Manta, R. S. Jacob, V. N. Gladyshev, S. Abdelilah-Seyfried, P. Selenko, A. Binolfi
The paper develops an NMR-based biosensor to measure MSRA and MSRB activities in vitro and in vivo, showing differences in substrate specificity between prokaryotic and eukaryotic MSRs.
bioRxiv · May 14, 2020Biophysics