BiochemistrybioRxiv
Expression and purification of Protease Activated Receptor 4 (PAR4) and analysis with histidine hydrogen deuterium exchange.
M. de le Fuente, X. Han, M. Miyagi, M. T. Nieman
This paper expresses and purifies PAR4, then uses His-HDX to analyze conformational changes upon thrombin activation, showing increased solvent protection for specific histidine residues.
DOI 10.1021/acs.biochem.9b00987