Pre-treatment of the clinical sample with Proteinase K allows detection of SARS-CoV-2 in the absence of RNA extraction
L. Mallmann, K. Schallenberger, M. Demoliner, A. K. Antunes Eisen, B. S. Hermann, F. H. Heldt, A. W. Hansen, F. R. Spilki, J. D. Fleck
This study shows that pre-treatment of clinical samples with Proteinase K allows SARS-CoV-2 detection without RNA extraction, achieving 91% agreement with standard RT-qPCR results.
bioRxiv · May 7, 2020 · PreprintMicrobiology