Monitoring RNA restructuring in a human cell-free extract reveals eIF4A-dependent and eIF4A-independent unwinding activity.
M. H. O'Sullivan, C. S. Fraser
This study uses a fluorescent duplex unwinding assay to show that eIF4A and eIF4A-independent mechanisms each contribute 50% to duplex unwinding in cell-free extracts, with the m7G cap promoting unwinding.
bioRxiv · Dec 14, 2022Biochemistry