Machine learning-based modulation of Ca2+-binding affinity in EFhand proteins and comparative structural insights into site-specific cooperative binding
M. Mazumder, S. Kumar, D. Kumar, S. Gourinath
The paper designs an EF-hand loop with high Ca2+ affinity using machine learning and validates it through mutagenesis, ITC, and crystallography, showing enhanced binding and altered oligomerization.
bioRxiv · Oct 4, 2021Biophysics