Strand-switching mechanism of Pif1 helicase induced by its collision with a G-quadruplex embedded in dsDNA
J. Valle-Orero, M. Rieu, P. L. T. Tran, A. Joubert, J.-F. Allemand, V. Croquette, J.-B. Boule
The paper develops a single-molecule assay to study Pif1 helicase resolving G-quadruplexes and reveals its strand-switching behavior affects G4 resolution efficiency.
bioRxiv · Jan 11, 2022Biophysics