Using Polycistronic gRNAs and a tRNA Cleavage System in CRISPR-Based Genome Editing of Toxoplasma gondii
Janessa Grech, Melanie Teng, Renfu Wang, Matthew A. Child
This paper improves CRISPR-based genome editing in Toxoplasma gondii by optimizing gRNA expression and introducing a tRNA-based polycistronic system for multiplexed editing.
bioRxiv · Oct 13, 2026 · PreprintMolecular Biology