Matthew A. Child

This paper improves CRISPR-based genome editing in Toxoplasma gondii by optimizing gRNA expression and introducing a tRNA-based polycistronic system for multiplexed editing.

CORe Designer is a CRISPR tool that allows protein-centric design of guide RNAs and repair templates for proteome engineering, streamlining targeted mutagenesis experiments.

CRISPR-TAPE is a protein-centric gRNA design tool that enables targeting of specific residues or amino acid types, improving efficacy and reducing computational time.